This book is not just a guide but a comprehensive resource designed to equip dental students, researchers, and practitioners with a deep understanding of biostatistics and its application in dentistry. This book provides a step-by-step guide from creating a file to entering data using IBM SPSS Statistics version 28. It covers descriptive statistics and data exploration through visualisation. Subsequently, the book delves into inferential statistics, including parametric tests (one-sample t-test, independent sample t-test, paired t-test, and one-way ANOVA) and nonparametric tests (one-sample Wilcoxon signed-rank test, Mann-Whitney U test, Wilcoxon paired signed-rank test, Kruskal Wallis test).